[Differentially expressed transcription factor-related genes in nasopharyngeal carcinoma and nasopharyngeal tissues].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University(2007)
Abstract
OBJECTIVE:To analyze the gene expression profiles of transcription factor-related genes in nasopharyngeal carcinoma (NPC) tissues and normal nasopharyngeal tissues using a cDNA microarray membrane for exploring the regulatory mechanism of differential gene express in NPC tissues.
METHODS:The total RNAs from 24 NPC tissues and 24 pooled normal nasopharyngeal tissues were reverse transcribed and labeled with alpha-(32)P-dCTP. The resultant cDNAs were hybridized to GF211 microarray, and the signals were analyzed by Pathway 4.0 software. RT-PCR was carried out to confirm the results.
RESULTS:Among the 1625 differentially expressed genes detected in NPC and nasopharyngeal tissues, 35 transcription factor-related genes were identified with either up- or down-regulation.
CONCLUSION:These differentially expressed transcription factor-related genes in NPC tissues might play a role in the regulation of NPC-related gene expression.
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Key words
nasopharyngeal carcinoma,nasopharyngeal tissues,genes,factor-related
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