Characterization of Nuclear Matrix Proteome of Drosophila melanogaster During Embryonic Development

The nucleus is an intricate structure containing many functional domains. Nuclear Matrix (NuMat), a non-chromatin scaffolding made of RNA and proteins, is believed to maintain this complex spatial organization. We have standardized procedure to prepare NuMat from Drosophila embryos by introducing several modifications in the published protocols and setting up several quality controls. We have established 2D profile of the NuMat proteome of Drosophila embryos and identified more than 150 proteins. While comparing the 2D profiles from different developmental stages, we noticed remarkable alterations in the composition of NuMat proteome during Drosophila development.