The determination of satraplatin in rat plasma and urine by graphite furnace atomic absorption spectrometry

OBJECTIVE To develop a rapid,accurate and sensitive method for the measurements of satraplatin in biological fluids of rats.METHODS Satraplatin in plasma and urine was analyzed by flameless atomic absorption spectrometry on an instrument Shimadzu AA-670 atomic absorption spectrophotometer equipped with a Shimadzu GFA-4A graphite furnace,and an ASC-60 autosampler.The detection wavelength was set at 265.9 nm.Plasma and urine samples were diluted with Triton X-100 before assays,fifty microliters of solution after preparation was injected to the system. RESULTS The linear ranges of satraplatin in plasma or urine were 0.25mg·L~(-1)~10.0mg·L~(-1).The correlation coefficient r~(2) was 0.9952 and 0.9878,and the regression equation were A=(0.2118)C-0.0548 and A=0.1556C-0.0042 for plasma and urine,respectively.The average recoveries were 89%~112% in plasma and 82%~100% in urine,respectively.The RSD values of interday and intra-day assays were lower than 8.9% and 6.4% respectably.CONCLUSION This method is suitable for the preclinical pharmacokinetic studies of satraplatin in rats.