The Types of siRNA Off-target Effects and the Strategies for Mitigation

Small interfering RNAs(siRNAs) can specific silence target genes,and are widely used to elucidate gene function,indentify drug targets and develop more specific therapeutics than are currently available.Off-target effects(OTEs) can complicate the interpretation of phenotypic effects in gene-silencing experiments and can potentially lead to unwanted toxicities.siRNAs OTEs include microRNA-like off-target effects,immune stimulation and saturation of the RNAi machinery.The types of off-target effects of siRNAs and methods to mitigate them was focused,to help enable effective application of RNAi technology.