The frequency-domain method reveals the dimeric structure of Na,K-ATPase.
Journal of fluorescence(1993)
摘要
Lucifer yellow and lissamine rhodamine sulfonyl hydrazine were used as the donor and the receptor, respectively, for Förster energy transfer measurements to determine the location of the β subunit in the native Na,K-ATPase from pig kidney. It was found that (1) the β subunits are located in one functional complex, i.e., the dimer (αβ)2 appears to be the functional complex of Na,K-ATPase, and (2) the β subunits in the functional enzyme complex in the membrane are not located next to each other but are rather well separated. The distance between fluorophores covalently attached to the β subunits was found to be 5.3 nm.
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关键词
Na,K-ATPase,frequency domain,Lucifer yellow,lissamine rhodamine sulfonylhydrazine,dimeric structure
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