[Expression of aldolase of Toxoplasma gondii and preparation of the polyclonal antibodies against this protein].

AIM:To produce the aldolase protein and its polyclonal antibody. METHODS:Aldolase gene was obtained from cDNA library by PCR amplification and subcloned to vector pET30a. The recombinant protein aldolase-His(6); was expressed in E.coli upon IPTG induction and then purified with affinity chromatography. The purified protein mixed with adjuvant was used to immunize SD rats to produce the polyclonal antibodies. RESULTS:The recombinant plasmid aldolase/pET30a was constructed successfully and expressed as a fusion protein aldolase-His(6);; Polyclonal antibody against aldolase-His(6); was obtained from rat, and antibody titer was 1:4 000. CONCLUSION:The purified protein aldolase-His(6); and its polyclonal antibodies were obtained, which may provide the foundation for the further studies on the function of aldolase.