Development Of The Model Of Pancreatic Beta-Cell Membrane Chromatography And Its Chromatographic Characteristics

A new model of pancreatic beta-cell membrane chromatography has been established by using a beta-cell membrane stationary phase (beta-CMSP) prepared by immobilizing the beta-cell membrane onto the surface of silica carrier. The protein level and K+, Na+-ATP enzymatic activity of the beta-CMSP were detected respectively. The surface characteristics of the beta-CMSP were tested by using the scanning electron microscope and surface energy spectrometer. In this model, the column (10 mm X 2 mm, I.D.) packed with beta-CMSP, 25 mmol/L ammonium sulfate buffer solution (pH 7.4) as mobile phase with the flow rate of 0.2 mL/min at 37 0.5 degrees C were used in the following studies. The retention characteristics of the sulfonylureas (gliquidone, glibenclamide, gliclazide and glipizide) were investigated under the chromatographic conditions above. The affinities of the sulfonylureas on beta-cell membrane and receptor will be expressed by using the logk' values (the logarithm of capacity factor of a solute) in the model. The correlation of the affinity with the pharmacological effect of the sulfonylureas was analyzed also.