UPLC-MS-MS法测定血液中5种吩噻嗪类药物
Chemical Analysis and Meterage(2012)
Abstract
建立了超高效液相色谱串联质谱法测定血液中5种吩噻嗪类药物的方法。血液样品经过沉淀蛋白后,在HSS SB C18色谱柱上,经乙腈–0.1%甲酸水溶液梯度洗脱分离,用液相色谱–串联质谱仪以电喷雾电离(ESI+)、多反应监测(MRM)方式进行分析。血液样本中异丙嗪、泰尔登、氯丙嗪、奋乃静、三氟拉嗪提取回收率分别为62%~65%,71%~97%,66%~107%,72%~95%,77%~96%,检出限分别为0.02,0.01,0.01,0.01,0.01 ng/mL。测定结果的相对标准偏差小于7%(n=6)。线性范围为0.05~20 ng/mL,相关系数大于0.999。该方法适用于血液中吩噻嗪类药物的分析。
MoreTranslated text
Key words
phenothiazine drugs,human plasma,super high performance liquid chromatography tandem mass spectrometry(UPLC-MS-MS)
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined