Collection of Drosophila Embryos for RNA Interference (RNAi).

INTRODUCTIONRNA interference (RNAi) is a powerful method for determining the role of specific genes during Drosophila embryogenesis. This protocol describes a method for collection of Drosophila embryos for RNA interference (RNAi) experiments. The embryos are collected in a simple, homemade apparatus, arrayed on prepared glass slides, and readied for injection. It is important to keep the embryos moist and oxygenated. Work expeditiously, because embryos should be injected within 30-60 min after collection.