拟南芥类锌指基因AT3G02790/AT5G16470纯合双突变的筛选及其表型分析

Journal of Northeast Forestry University(2012)

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Abstract
以拟南芥T-DNA插入突变体AT3G02790和AT5G16470为亲本,通过人工杂交技术获得杂合体后,结合基因分离定律和PCR技术筛选出拟南芥纯合双突变株,并对拟南芥纯合突变体的表型进行了初步分析,结果显示:突变体与野生型植株表型无明显差异,推测该基因为功能未知的新基因。
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Key words
Double-mutant,Arabidopsis thaliana,Stress,Phenotypes,Crossing
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