Effects of recombinant human VEGF-C and VEGF-D on proliferation of human lymphatic endothelial cells

Purpose To investigate the effects of VEGF-C and VEGF-D on proliferation of human lymphatic endothelial cells and explore mechanism of lymphangiogenesis induced by VEGF-C and VEGF-D.Methods The human lymphatic endothelial cells were cultured in vitro.Cells had been purified at the third passage.After stimulation with recombinant human VEGF-C(rhVEGF-C),rhVEGF-D,rhVEGF-C+rhVEGF-D,the proliferation of cells was compared in each group at last.Results The human lymphatic endothelial cells cultured in vitro has significantly proliferated after adding reagents.The proliferation rate of VEGF-D group was(54.2±4.35)%,the proliferation rate of VEGF-C group was(62.5±5.67)% and in VEGF-C+VEGF-D group it was(74.3±5.08)%.They all had significant difference to the control group(P0.05).Conclusion The supernatant of human recombinant VEGF-C and human recombinant VEGF-D cell cultured promote proliferation of lymphatic endothelial cells.