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硫代硫酸钠在四甲基联苯胺贮液保存中的应用

Journal of China Agricultural University(2006)

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Abstract
为解决试剂四甲基联苯胺(TMB)贮液在储藏时容易变色的问题,本研究改良了TMB贮液的配方,即在原有每毫升TMB贮液中加入3μL 2 mg/mL的抗氧化剂五水硫代硫酸钠(Na2S2O3.5H2O)溶液。试验结果表明,新配方TMB贮液至少能在4℃下贮存3个月不变色且不影响显色效果。同时,对照原有配方中已经变色的TMB贮液,发现根据变色深浅,每毫升中加入2~6μL 2 mg/mL Na2S2O3.5H2O溶液,3~6 h后会变回无色,颜色消失的TMB贮液可重新利用而不影响显色效果。
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3,3′,5,discolor,sodium thiosulfate pentahydrate,ELISA,5′-tetramethylbenzidine(TMB)
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