Occurrence of 13(S)-Hydroxyoctadecadienoic Acid in Biological Samples
Prostaglandins(1997)
摘要
The oxygenated metabolite of linoleic acid, 13(S)-hydroxyoctadecadienoic acid has recently been shown to play a role in cellular regulation. To detect this molecule in biological systems, we recently developed a specific polyclonal antibody. Using this antibody, we report the presence of 13(S)-hydroxyoctadecadienoic acid in human urine, cell culture media, and untreated goat serum for the first time by a specific, sensitive, and rapid enzyme immunoassay. Furthermore, the enzyme linked immunosorbent assay data are verified by gas chromatography/mass spectrometry analysis of the same samples.
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关键词
13-Hydroxyocadecadienoic acid,dietary fatty acids,linoleic acid,immunoassay,oxidation
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