Characterization and the Anti-tumor Effect of Doxorubicin Flexible Liposome in vitro

Objective: To evaluate the potential application of flexible nanoliposomes containing membrane softening agent in intravenous administration by comparing doxorubicin(DOX) load capability and tumor cell inhibition of different nanoliposomes.Methods: Traditional liposomes(DOX-CL) and flexible nanoliposomes(DOX-FNL) were prepared by film hydration method with DOX as a model drug.Encapsulation efficiency of liposomes were investigated by Dextran gel chromatography column method.The hydrodynamic size,zeta potential and polydispersity index(PDI) of liposomes in different pH condition and media were characterised by particle size analyzer.The drug release of two liposomes in vitro were detected with membrane dialysis method.The human breast cancer MCF-7cell viability of two liposomes were investigated with MTT assay.The cell cycle changes of MCF-7 cells induced by DOX-CL and DOX-FNL were detected by flow cytometry.Results The encapsulation efficiency of DOX-FNL was higher than DOX-CL,and the DOX-FNL had more excellent suspension stability in neutral pH conditions,water,physiological saline,PBS and RPMI-1640 culture medium with/without 10% FBS.The release rate of two liposomes was no significant difference(P>0.05) in different pH conditions.The cytotoxic detection results showed that the inhibition of MCF-7 cells was hardly different between DOX-CL and DOX-FNL,while tumor cell killing effect of DOX-FNL was more endurable than DOX-CL(P<0.05).The FCM detection results showed that the cell cycle of MCF-7 arrested in G0/G1 phase by DOX-FNL was similar as the DOX-CL,moreover,DOX-FNL is more effective than DOX-CL(P<0.05).Conclusion DOX-FNL has higher drug encapsulation efficiency and stability than DOX-CL.DOX-FNL can affect the cell cycle,and its drug action time is significantly higher than DOX-CL.