兔肌腱细胞原代培养方法研究

Medical Journal of the Chinese People's Armed Police Forces(2011)

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Abstract
目的探索兔肌腱细胞的培养方法,为临床人工肌腱等组织工程研究提供理论依据。方法用消化贴块法培养兔体肌腱细胞,用倒差显微镜及共聚焦显微镜观察细胞生长及形态特征。结果兔肌腱细胞在体外呈长梭形,胞质均匀透明,细胞核均匀,汇合后呈平行致密排列并有层叠等特征,传代后生长特征仍保持稳定。结论本实验成功培养了兔肌腱细胞,为肌腱组织工程学种子细胞的来源研究提供了一定的借鉴。
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Key words
cell culture,primary,tendon cells
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