A useful ELISA system for human liver-type arginase, and its utility in diagnosis of liver diseases11Abbreviations used: ELISA, enzyme-linked immunosorbent assay; HRP, horseradish peroxidase; AST, aspartate aminotransferase; ALT, alanine aminotransferase; LDH, lactate dehydrogenase; SDS, sodium dodecyl sulfate; SDS-PAGE, polyacrylamide gel electrophoresis in the presence of SDS.

Masaki Ikemoto,Shoji Tsunekawa,Masaaki Awane,Yoshihiro Fukuda,Hiroshi Murayama,Makoto Igarashi,Atsuo Nagata,Yasunari Kasai,Masayuki Totani

Clinical Biochemistry（2001）

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摘要

Objectives: To develop a new ELISA system for liver-type arginase using monoclonal antibodies against the enzyme, and to verify the utility of the arginase in diagnosis of hepatic disorders.

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关键词

Liver-type arginase,ELISA,Liver injury,Hemoglobin,Monoclonal antibody,Liver disease,Urea cycle,Erythrocytes,Kidney

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