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HPLC测定野木瓜中木犀草素的含量

Chinese Journal of Experimental Traditional Medical Formulae(2013)

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Abstract
目的:建立野木瓜中木犀草素的含量测定方法。方法:Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),流动相乙睛-0.1%磷酸溶液(26∶74),流速1 mL.min-1,检测波长350 nm,柱温室温。结果:线性范围0.067 75~2.168μg(r=0.999 9),平均回收率为99.25%,RSD 1.65%,精密度RSD 2.22%,重复性RSD 1.24%,稳定性RSD 2.58%。结论:方法稳定、可靠,可作为该药材的质量控制方法。
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Key words
HPLC,Caulis et Folium Stauntoniae,luteolin
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