Anti-Inflammatory Mechanism of the Important Ingredient of Salvia miltiorrhiza Bunge-Tanshinone II-A in RAW264.7 Cell Line

Objective: To research the anti-inflammatory mechanism of tanshinoneⅡ-A, which is an important ingredient in Salvia miltiorrhiza Bunge. Methods: RAW264.7 cell lines were inhibited by tanshinoneⅡ-A with different concentration as target cell. When RAW264.7 were inhibited for 24, 48 h, used MTT assay and semi-quantitative RT-PCR. Results: According to MTT assay, IC50 of the anti-inflammatory is 43.2 μmol/L; in semi-quantitative RT-PCR experiment, tanshinoneⅡ-A inhibit the action of phospholipases A2. Conclusion: The results also demonstrate the action of anti-inflammatory mechanism is performed by inhibiting the action of phospholipases A2.