Southern and dot blot analysis of DNA from formalin-fixed, paraffin-embedded tissue samples from colonic carcinomas
Virchows Archiv. B, Cell pathology including molecular pathology(1990)
摘要
Summary Two extraction methods for the isolation of DNA from formalin-fixed, paraffin-embedded tissue samples from colonic carcinomas
were compared. The processed DNAs were compared with DNAs from fresh specimens of the same tumors. The two extraction methods
gave similar results. Formalin-fixation and paraffinembedding irreversibly denatured DNA and consequently decreased the extraction
yield and interfered with the quantitative measurement of DNA.
Southern blot and dot blot analysis of processed and native DNA was performed using a c-myc and an actin probe. The results
show that for Southern analysis processed DNA can be used but, due to the generation of random breaks, the restriction fragments
have to be small. Furthermore, the fixation-induced crosslinking of DNA appears to hamper hybridization. For these reasons
processed DNA can be analyzed better by dot blot rather than Southern blot hybridization.
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关键词
colon cancer,dna degradation,formalin fixed tissue,southern blot,dot blot,Southern blot,Dot blot,Formalin fixed tissue,Colon cancer,DNA degradation
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