高海拔地区牦牛心肌骨骼肌ATP酶活性的测定

Chinese Journal of Veterinary Medicine(2012)

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Abstract
为了探讨高原动物对高原低氧的适应机制,采用标准ATP酶测试盒对青海省(平均海拔4 000m)16头成年牦牛心肌、骨骼肌ATP酶活性进行了测定和分析。结果:Na+-K+-ATP,Mg2+-ATP,Ca2+ATP和Ca2+-Mg2+-ATP 4种酶活性在骨骼肌线粒体中分别为3.65±1.31(μmolP/mg蛋白.h,下同),2.70±1.36,2.87±1.23和4.08±1.30;在心肌线粒体中分别为4.44±1.19,2.42±0.87,2.29±0.57和3.62±0.92;在骨骼肌组织中分别为2.02±0.80,1.54±0.36,1.69±0.91和2.02±0.96;在心肌组织中分别为1.41±0.67,1.04±0.51,1.24±0.54和1.42±0.60;骨骼肌、心肌线粒体中4种ATP酶活性均显著高于骨骼肌、心肌组织(P<0.05)。
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Key words
mitochondria,cardiac muscle,Yak,ATP,skeletal muscle
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