Effects of Didang Qigui decoction on streptozotocin induced diabetic cataract in rats.

Objective To observe the effect of Didang Qigui decoction induced diabetic rats lens,lens epithelial cells and the level of HSP70 effects on STZ.Methods a total of 10 SD rats into normal control group,the other rats by intraperitoneal injection of streptozotocin(STZ) 60 mg / kg,the model of diabetic rats.Selected into the model animal and 55 rats were randomly divided into model group,gliclazide group,Didang Qigui decoction group,Didang Qigui decoction high dose group,Didang Qigui decoction and Diamicron group,to give the corresponding intervention respectively.In Experiment 2,4,6,8 W under slit lamp examination the opacity of the lens epithelial cells;morphology changes of lens to observe 8W under the microscope,and the detection of lens epithelial cell(LEC) expression in HSP70.Results the blank control group rat lens remain transparent integrity,lens model group rats gradually so completely cloudy,the rest of the rats in the treatment group lens opacity degree from heavy to light are Didang Qigui decoction high dose group,Didang Qigui decoction group,gliclazide group,Didang Qigui decoction and Diamicron group;HE staining morphology of lens epithelial cells also have different change;in the blank control group rats were used as standards,gradually increased the expression of HSP70 in LEC model group lens,the treatment group of Didang Qigui decoction large doses of the positive expression rate of HSP70 was high on the Hong group,arrived when the positive expression of Didang Qigui decoction group,Didang Qigui decoction and Diamicron group HSP70 the rate,the difference was significant(P < 0.05).Conclusion Didang Qigui decoction by lowering blood sugar,reducing sugar,reduce the accumulation of metabolites crystalline and osmotic pressure in vivo so as to effectively protect the lens damage in experimental diabetic rats in the crystalline body,and inhibit the development of cataract in diabetic rats,has a preventive effect on early diabetic rat cataract.