Radioiodination of mouse anti-III β-tubulin antibodies and their evaluation with respect to their use as diagnostic agents for peripheral neuropathies
Applied Radiation and Isotopes(2008)
摘要
The monoclonal antibody TU-20 and its scFv fragment were radiolabeled with 125I in order to develop new imaging agents against the specific neuronal marker III β-tubulin. The reaction via chloramine-T using thiosulfate as a stopping reductant was determined as the most convenient way for radioiodination. The preserved immunological properties of radioiodinated species were estimated by ELISA, electrophoresis, and immunohistochemistry with autoradiography. Biodistribution studies revealed a different behavior of radioiodinated TU-20 and its scFv.
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关键词
III β-tubulin,Radioiodination,125-Iodine,MAb,scFv,Peripheral neuropathies
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