褐蘑菇水溶性多糖抗氧化活性研究

Natural Product Research and Development(2012)

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Abstract
采用水提醇沉以及sevag去蛋白的方法获得褐蘑菇水溶性多糖(WPPA).通过测定还原力、超氧阴离子清除率、羟基自由基清除率和抑制H2O2诱导红细胞氧化溶血实验评价WPPA抗氧化活性.结果表明:WPPA具有较强的还原力,对O2-和·OH具有较强的清除作用,IC50分别为527 μg/mL、310 μg/mL;对H2O2诱导红细胞氧化溶血及MDA生成有很强的抑制作用,IC50分别为700μg/mL和541μg/mL.说明WPPA在一定浓度内具有较强的抗氧化能力.
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Key words
Agaricus bisporus,polysaccharides,antioxidant activity,erythrocyte hemolysis
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