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抗虫杀菌融合基因表达载体的构建及微束激光转化植物研究

ZHANG Nan,CHEN Zheng-hua,LIU Gui-zhen,CHEN Ling-na,QU Yan-ying

Acta Laser Biology Sinica（2007）

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Abstract
将苏云金芽孢杆菌伴孢晶体蛋白的基因(Bacillus thuringiesis,简称Bt)通过甘氨酸接头(Gly4Ser)3与一种人工合成的抗菌肽(antimicrobial peptides,AMP)基因与相融合,编码一种新的杀虫,并具有抗菌的蛋白。把融合基因(NAMP-Bt)连接到原核表达载体pET-28a和植物表达载体pBI-121上,经过限制性酶切分析和PCR鉴定,结果表明含有融合基因的原核和真核重组表达质粒均已构建成功,并将该融合基因转入烟草,已获得抗性小植株。
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Key words
analysis of restriction enzyme,PCR analysis,antimicrobial peptides gene,plant expression vectors,Bacillus thuringiesis,prokaryotic expression vectors
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