Production of Large-Ring Cyclodextrins Composed of 9 ~ 21 a-D-Glucopyranose Units by Cyclodextrin Glucanotransferase — Effects of Incubation Temperature and Molecular Weight of Amylose

Cyclodextrin glucanotransferase (CGTase) is the only enzyme that can produce large-ring cyclodextrins (LR-CDs) composed of 9 similar to 21 alpha-D-glucopyranose units (CD9 similar to CD21) In this study, two amyloses with different average molecular weights (14.6 and 280 kDa) were incubated with CGTase at three different temperatures, and the yield and composition of the LR-CDs were examined. The composition was not significantly affected by the size of amylose used, but was greatly affected by the reaction temperature.