Influence of variation angiogenesis in acute hind limb ischemia diabetic rat model by using angiography method

Objective To evaluate the model of diabetic rats′ acute hind limb ischemia through the method of angiography,and to explore the influence of variation angiogenesis for contributing the model.Methods Thrity male Wistar rats after fed with high-lipid food were intra-peritoneal injected streptozocin,streptozotocin(STZ,40 mg/kg) to evoke diabetic model.Femoral artery of the diabetic rats model were ligated to cause acute hind limb ischemia.The model rats were fed 28 days,the angiogenesis was observed by angiography method on the 28 days.Results There were 25 rats(83.3%) with the fasting blood glucose more than 16.8 mmol/L,the hind limb was obviously ischemia(P0.05) observed by eye on first 7 days after ligation,and the ischemia almost recovered to condition of preoperative after 28 days,muscle of ischemic hind limb slightly atrophy compared with the other side,but movement was convenient.After 28 days,arteries branch from internal iliac artery thickened evidently,blood vessels of the distal end raised compensatory,providing the area of knee joint and the blow.Conclusion There are branches from internal iliac artery to participate the blood-supply,the variation have influences on establishing the ischemia model;by using angiography can discovery this kind of variation,and accurately evaluate the diabetic hind limb ischemia and the compensatory changing of blood vessels.