Preliminary Study of Radix Asparagi Extracts as Inactived Influenza Vaccine Adjuvant

Objective To explore the adjuvant effect of Radix Asparagi extracts on inactivated influenza virus vaccine and to optimize the dose of them by quadratic orthogonal rotation combination design.Methods Different dosages of Radix Asparagi extracts mixed with different dosages of inactivated influenza virus H3N2 vaccine were injected intramuscularly into mice.And mice immunized respectively with inactivated vaccine alone,aluminium-adjuvanted inactivated vaccine and physiological saline which were control groups.Each group of mice was immunized twice at 3 week intervals.Two weeks after the boost,blood samples were collected for measurement of serum hemagglutination inhibition(HI) titers.And further study on the dose of Radix Asparagi extracts and inactivated vaccine HA was evaluated by quadratic orthogonal rotation combination design with HI titers as response value.The data was analyzed by SAS9.1 software.In addition,the dose of Radix Asparagi extracts and inactivated vaccine HA with the optimal response value were defined.Result Radix Asparagi extracts significantly enhanced the HI titers,which was higher than those of aluminium.And when the dosage of Radix Asparagi extracts and inactivated vaccine HA respectively were 0.1mg and 4.345 ?g,the optimal response value(HI titer) obtained was 5 092.Conclusion Radix Asparagi extracts can be used as a potential adjuvant of inactivated influenza virus vaccine.