37 MORPHOLOGICAL FEATURES OF SPERMATOZOA (LIKE “HEAD-IN-TAIL” ANOMALY) AND “COMET ASSAY” FROM PATIENTS WITH DECLINED QUALITY OF SEMEN

the mean age was 33.29 years. The clinical trial was conducted in Ivanovo clinic-diagnostic center of municipal hospital No. 8. Statistical analysis was performed with Microsoft Excel v.12.0 and Statistica v6.1. Men applied to the hospital with infertility and diagnosed the autoimmune infertility (MAR%IgG and/or MAR%IgA 50%) were included in this trial. Spermogram results: the mean spermatozoa with adhesive antisperm antibodies (ASA) type IgG was 54%, and type IgA 60%; mean spermatozoa A 9%, mean spermatozoa B 16%; total amount of spermatozoa in ejaculate A 22.46mil, total amount of spermatozoa in ejaculate B 32.98 mil; mean spermatozoid speed was 8.83mm/s, mean viscosity 3.63 cm. All patients were treated with Longidaza. All the same indicators were evaluated after 3 months of treatment. The mean spermatozoa with ASA type IgG significantly decreased to 15% (p = 0.0016), type IgA to 14% (p = 0.001). Mean spermatozoa A was 11%, B 21%, A+B 32%, total amount of spermatozoa in ejaculate A 30.08mil, total amount of spermatozoa in ejaculate B 30.35mil; mean spermatozoid speed was 9.67mm/s, mean viscosity 3.18 cm. No side or adverse effects were registered by the patients during the treatment. Therefore, statisticallyvalid decrease of spermatozoa with ASA type IgG mean 51%, and type IgA 37%. Qualitative and quantitative spermogram indicators improved, but not statistically-valid, apparently, this is connected with small sample size.