Quenching Mechanism of Mechanically Compressed Green Fluorescent Protein Studied by CASSCF/AM1

Qi Gao,Katsuori Tagami,Masamichi Fujihira,Masaru Tsukada

JAPANESE JOURNAL OF APPLIED PHYSICS PART 2-LETTERS & EXPRESS LETTERS（2014）

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摘要

Atomic force microscopy (AFM) compression simulation approach together with "CASSCF/AM1 ONIOM" model calculation method is used for investigating the quenching mechanism of the luminescence in green fluorescent protein (GFP). Our results show that the electronic properties and steric conformations of the chromophore's neighboring residues strongly influence the photo decay behaviors in GFP.

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关键词

afm,gfp,quenching,CASSCF,ONIOM

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