XAGE-3,A Primate Specific Gene Transgenic Research

Objective To assess the XAGE-3 homologous characteristics in primate and rodent species,and to analyze the XAGE-3 biological function in transgenic mice.Methods Based on the databases Homologene and Taxplot,to compare the homologous characteristics in different genomes,to clone XAGE-3 gene from human placenta,to construct the eukaryotic expression vector pCDNA3.1(+ ),to create transgenic mice by microinjection,to identify the genotyping and the expression of transformed gene by genomic PCR and reverse transcription PCR,and to check the proliferation of spermatocytes in transgenic mice and control ones by BrdU labeling.Results The XAGE-3 gene had homologs in Homo sapiens,Pan troglodytes and Macaca mulatta,not in Mus musculus and Rattus norvegicus.XAGE-3 gene expression was detected in the testis in 3 of 5 transgenic lines,and in 2 lines chosen for phenotype analysis.The expression was detected in the intestine,thymus and testis.More spermatocytes were labeled by BrdU in transgenic mice compared with that in the negative control.Conclusion Primate species specific gene XAGE-3 may affect the proliferation of spermatacytes.