Partial Purification and Characterization of an Enzyme Releasing 2-Sulfo-.ALPHA.-L-fucopyranose from 2-Sulfo-.ALPHA.-L-fucopyranosyl-(1.RAR.2) Pyridylaminated Fucose from a Sea Urchin, Strongylocentrotus nudus.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY（2014）
Abstract
We purified an enzyme hydrolyzing 2-sulfo-alpha-L-fucopyranose pyridylaminated 2-sulfo-alpha-L-fucopyranosyl-(1-->2)-fucose from the acetone powder of the digestive tract of a sea urchin. The enzyme was purified 307-fold with an overall recovery of 1.63% by ammonium sulfate precipitation, cation exchange chromatography, and gel filtration chromatography. The enzyme is useful for the structural analysis of sulfated fucan.
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Key words
2-o-sulfated fucose releasing enzyme,sulfated fucose,fucoidan,PA-oligosaccharide,sea urchin
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