混菌固态发酵豆粕制备多肽饲料培养基的优化

Cereal & Feed Industry(2013)

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Abstract
采用枯草芽孢杆菌CGMCC1.0892和米曲霉CGMCC2.0951混合固态发酵豆粕,研究发酵产物中多肽的含量。运用Minitab 16软件,建立了大豆多肽含量与麸皮添加量、腐植酸钠添加量、磷酸氢二钾添加量三个影响因子的回归方程,并得到以大豆多肽含量为响应值的响应面图和等高线图。确定了枯草芽孢杆菌和米曲霉混合发酵豆粕制备多肽饲料的培养基成分为:腐植酸钠添加量1.75%,麸皮添加量5.83%,磷酸氢二钾添加量0.74%,在此条件下大豆多肽的理论含量可达12.65%。
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Key words
Aspergillus oryzae,soybean peptides,soybean meal,solid state fermentation,Bacillus subtilis,response surface methodology
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