HPLC法测定肿节风药材中异秦皮啶和迷迭香酸的含量
Journal of Nanchang University(Natural Science)(2010)
Abstract
建立了肿节风中异秦皮啶和迷迭香酸含量的测定方法.以乙腈-0.1%磷酸溶液(20:80,V/V)为流动相,流速为1.0 mL/min,柱温35 ℃,UV检测波长为342 nm.样品经0.45 μm微孔滤膜过滤后,按选定的色谱条件进行分析,异秦皮啶和迷迭香酸分别在0.011~2.214 μg/mL、0.022~2.198 μg/mL范围内线性关系良好,相关系数均为0.999 0.方法的加标回收率为102.57%~102.94%.该方法灵敏快速,操作简便,重现性好,可用于肿节风药材的质量控制.
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Key words
HPLC,Sarcandra glabra,isofraxidin,rosmarinic acid
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