Screening colorectal cancer tumor-related genes with gene chip.

Objective To investigate the different tumor-related genes expression in colorectal cancer.Methods The expressed differentially genes between colorectal cancer and normal colorectal tissues were analyzed with gene chip(contained 8 064 human target genes),and of 4 genes were verified by reverse-transcription polymerase chain reaction(RT-PCR).Results The 1 807 genes expressed differentially in colorectal cancer,and 936 genes unregulated and 871 genes down regulated;The different expression of oncogenes and tumor suppressor genes were 57,which 36 genes unregulated and 21 genes down regulated.Among the 1 807 expressed differentially genes,the results of RT-PCR to verify 4 pairs of differential expression genes were conformed to gene chip.Conclusion It is demonstrated that colorectal cancer development is related to differential expression of multiple proto-oncogenes and tumor suppressor gene.