Detection and quantitation of HBV DNA in the WHO International Standard for HIV-1 RNA (NIBSC code: 97/656).
Journal of Virological Methods(2004)
摘要
Nucleic-acid amplification technology (NAT) assays have been implemented for HCV and HIV-1 in the United States, and many parts of Europe, Australia and Asia. Nucleic acid detection assays utilize many different technologies, and the WHO International Standards for nucleic acid tests are widely used to compare them. Currently, several laboratories are developing an assay for simultaneous detection of HCV RNA, HIV-1 RNA and HBV DNA. In the course of such development it was observed that the WHO International Standard for HIV-1 RNA (97/656) was positive for HBV DNA. In this report we confirm the presence of HBV DNA in the HIV-1 international standard through the qualitative Procleix®-Ultrio™ assay. Further, using the TaqMan® technology, through quantitative Bead Capture-TaqMan assay, we report that ∼1000IU/ml dilution of HIV RNA contains ∼4500IU/ml of HBV DNA.
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关键词
WHO International Standard for HIV-1 RNA,Procleix® HIV-1/HCV assay,Procleix®-Ultrio™ assay,Procleix® dHBV assay,TaqMan® technology,Quantitative Bead Capture-TaqMan HBV assay
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