Novel Method For The Study Of Human Epithelial Ion Transport.

Mammalian duodenum carries out net HC0 3 secretion with a steep proximal-to-distal gradient.The cellular mechanims involved in epithelial HC0 3 transport involve acidlbase transporters.The hypotheses of this study was that since the epithelial Na+/H + exchangers (NHE) are present on human villus epithelial cells, that inhibition of their activity will increase luminal HCO J by decreasing HC0 3 absorption .Duodenal mucosal bicarbonate secretion (DMBS) was measured after an overnight fast in 5 healthy male subjects (ages 32-55 yrs).The proximal 3 em of duodenum