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鸭肝炎病毒Ⅰ型VP3基因的克隆及原核表达

LIU Jia-sen,GAN Yi-di,JIANG Qian,MENG Qing-wen,HAN Ling-xia,SI Chang-de,LIU Di,QU Lian-dong

Progress in Veterinary Medicine（2009）

Cited 7|Views16
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Abstract
参照鸭肝炎病毒Ⅰ型(DHV-Ⅰ)的基因组序列设计并合成了1对引物。通过RT-PCR方法扩增获得了DHV-Ⅰ病毒161/79/V结构蛋白VP3基因,并将VP3基因片段插入pGEX-6p-1表达载体,转化E.coli Rosetta-gami(DE3)pLysS。SDS-PAGE分析表明,经用0.1mmol/LIPTG于16℃诱导表达后,菌体裂解产物有特异的、分子质量约52ku的目的蛋白条带。Western-blotting检测表明,表达产物与DHV-Ⅰ阳性血清发生特异性反应,具有良好的反应原性。
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Key words
cloning,duck hepatitis virus typeⅠ,prokaryotic expression,VP3 gene
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