Effects and Mechanism of Sibiraea Angustata on Lipid Metabolism in Hight-fatted Rats

OBJECTIVE:To investigate the effect and mechanism of Sibiraea angustata on lipid metabolism in hight-fatted SD rats.METHODS:After the obese model was built,Sibiraea angustata was administrated intragastrically to obese rats for 8 weeks. Peeled off fat around kidneys and made pathological tissue sections. The number and size of adipocytes were detected. The levels of adiponectin, adipoR2, AMPK, and PPARgamma mRNA in adipose tissue were detected by RT-PCR. AMPK protein expression in adipose tissue were detected by Western Blot.RESULTS:Compared with the model group, the diameter of adipocytes were reduced while the number increased after adminiseration of Sibiraea angustata for 8 weeks. The levels of adiponectin, adipoR2, AMPK and PPARgamma mRNA were increased siginficantly. The expression of AMPK protein was also up-regulated significantly.CONCLUSION:Sibiraea angustata has anti-obesity effect. The mechanism may be related to the adiponectin signal transduction pathway.