Studies on PCR Primer Screening of Genetically Modified Plants and Derived Products

A method was established to screen PCR primers for detection of genetically modified plants and derived products.The transgenic soybean Roundup Ready standard reference was taken as experiment material.Every primer could amplify its product specifically from the standard reference after optimization of specificity PCR efficiency and melting curves.The primer RRS2 was proved to be the best primer for specific validation of transgenic soybean Roundup Ready using fluorescent quantitative PCR by comparison of Ct values and melting curves among the five specific Primers.