Genetic Diversity among Wild Resources of the Genus Boehmeria Jacq. from West China Determined Using Inter-simple Sequence Repeat and Rapid Amplification of Polymorphic DNA Markers

Ramie (Boehmeria nivea L.Gaud) is planted as an important fiber crop hi OWL Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used for the first time for die detection of genetic polymorphism of 37 ramie accessions (29 wild genotypes and 8 commercial varieties) collected front various geographical regions of West China. The objectives of 06 study were: 1) to examine the genetic diversity, of four specks in genus Boehmeria Jacq.: B. clidemioides var diffusa, B. nivea L. Gaud B. longispica Steud, and B. macrophylla Hornem; and 2) to determine the genetic distance of the four species by these Methods. Laportea cuspidata (Wedd.) Friis was used as an outgroup species. The results showed that 375 (17 monomorphic fragments) strips were amplified by 31 RAPD primers while 266 00 monomorphic fragments) strips by 18 ISSR primers. On the average, each RAN) and ISSR primer amplified 12.1 and 14.8 strips, respectively. Based on the appearance of the marker, the genetic relationships were analyzed using unweighted pair-group method (A arithmetic average cluster analysis (UPGMA) and We genetic Dice coefficients were calculated. Clustering analysis indicated that the 37 accessions were classified into four clusters which belong to 3 sections (including Section Phyllostachys, Section Tilocnide and section Duretia). The overall grouping pattern of clustering Corresponded well with traditional botanical taxonomy. Principal component. analysis PCA) confirmed the patterns of genetic diversity observed among the species. These results suggested that RAN) and ISSR were efficient approaches suitable for taxonomic analysis of ramie wild materials. The results provided valid guidelines for collection, conservation , and characterisation of Boehmeria genetic resources.