The Effect of Ginkgo Biloba on the Infiltration of Eosinophil in Airway of Asthma Rats

Objective: To study the role of Extract of ginkgo biloba(Egb) in regulating the activity of NF-κBp65 and Eotaxin protein of asthma rats,and in effecting the infiltration of eosinophil within airway.Method: Thirty-six Sprague-Dawley(SD) rats were divided into three groups of 12 each.The asthmatic model was established by ovalbumin(OVA) injection and inhalation.Group E were pretreated with Egb injection with 8mg·kg-1·d-1 for 14 days.The protein NF-κBp65 and Eotaxin in lung tissue were assessed by using immunohistochemisty.The numbers of EOS and the EOS% in BLAF were counted by different count fluids.The condition of airway inflammation were observed by pathology.Results: Twenty-four hours after the last antigen challenge,the protein expression of NF-κBp65(0.256±0.063)and Eotaxin(0.197±0.055) in group A were significantly increased compared with group C(0.034±0.008, 0.010±0.005 p0.01,respectively).The protein expression of NF-κBp65(0.062±0.014)and Eotaxin(0.115±0.010)in group E were significantly decreased compared with group A(p0.01,respectively).The numbers of EOS and the EOS% in BLAF of group E were significantly decreased compared with group A(p0.01,respectively).The conditions of airway inflammation of group E were significantly decreased compared with group A under light microscope.Conclusion: In asthmatic rats,the protein of NF-κBp65 and Eotaxin were increased significantly,Egb could inhibit the expression of the protein of NF-κBp65 and hold down the transcription of Eotaxin,and then reduce the infiltration of eosinophil within airway and decrease the condition of airway inflammation.