Inhibition of vascular endothelial growth factor (VEGF)-induced endothelial proliferation, arterial relaxation, vascular permeability and angiogenesis by dobesilate.
European Journal of Pharmacology(2011)
Abstract
Vascular endothelial growth factor (VEGF) is a key factor in angiogenesis and vascular permeability which is associated with many pathological processes. 2,5-hydroxybenzene sulfonate (DHBS; dobesilate) is a small molecule with anti-angiogenic activity that has been described as an inhibitor of fibroblast growth factors (FGF). The aim of the present study was to evaluate the effects of DHBS on VEGF-induced actions. The effects of DHBS were evaluated on VEGF-induced proliferation in human umbilical vein endothelial cells (HUVEC) and rat aorta relaxation, as well as on in vivo VEGF-induced skin vascular permeability and neovascularization in rats. DHBS at 50 and 100μM concentration significantly inhibited the proliferation of HUVEC induced by VEGF (10ng/ml), without significantly affecting HUVEC proliferation in the absence of VEGF. Rapid VEGF-induced activation of Akt in HUVEC was also prevented by DHBS (100μM). Additionally, DHBS (2μM) specifically inhibited the relaxation of rat aorta induced by VEGF (0.1 to 30ng/ml), but not endothelium-dependent relaxation to acetylcholine (1nM to 10μM). The in vivo enhancement of vascular permeability caused by VEGF injection (50μl at 10ng/ml) in rat skin was also inhibited by DHBS co-administration (200μM) (74.8±3.8% inhibition of dye extravasation). Administration of DHBS (200mg/kg/day; i.p.) also reduced VEGF-induced angiogenesis in vivo. DHBS inhibits main responses elicited in vitro and in vivo by VEGF. As a dual antagonist of VEGF and FGF activities, DHBS could be of therapeutic interest in the treatment of diseases related to VEGF/FGF overproduction and excessive angiogenesis.
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Key words
VEGF,FGF,Akt,Angiogenesis,Vascular permeability,Endothelium,Proliferation,Dobesilate
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