复合酶提高黄柏水提效率的关键技术研究
Journal of Emergency in Traditional Chinese Medicine(2013)
Abstract
目的探讨提高黄柏水提工艺效率的方法。方法在黄柏水提工艺程序中,加入纤维素酶-果胶酶-半纤维素酶组成的复合酶,按均匀试验设计考察5因素10水平,用SAS8.2统计分析,测定过滤速度及水提液的浸出物变化作指标,考察酶解工艺条件。结果每克黄柏取相当于酶活力1万U的纤维素酶,0.5万U的果胶酶,0.1万U的半纤维素酶,调温度37℃,pH为3.9的酶解条件下得到的黄柏水提液,分离速度提高5.64倍,黄柏碱与小檗碱收率分别提高2.17、1.14倍。结论应用复合酶可提高黄柏水提工艺的生产效率。
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Key words
Water-extracting technology,Separation speed,Cortex Phellodendri Chinensis,Complex enzyme
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