贴壁细胞在载玻片爬片的新方法
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology(2009)
Abstract
目的:对传统细胞爬片方法进行改良。方法:利用1ml蓝枪头和载玻片制作,在载玻片上种植细胞制成细胞爬片,进行后续染色。结果:细胞直接爬于载玻片上,未发现细胞污染和增殖受限。与传统爬片相比细胞脱落少。结论:与传统爬片染色方法相比具有用材简单、价格低廉、爬片效果好、操作方便等优点。
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Key words
cell culture,cell growing on the glass slide
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