Inhibitor of β-d-glucosidase and endo-1,4-β-d-glucanase produced by Aspergillus fumigatus IMI 255091

Inconsistencies in assays of fermentation broths of Aspergillus fumigatus IMI 255091 were observed for endo-1,4-β-d-glucanase [1,4-(1,3;1,4)-β-d-glucan 4-glucanohydrolase, EC 3.2.1.4] and β-d-glucosidase (β-d-glucoside glucohydrolase, EC 3.2.1.21). Dilution of the original sample appeared to enhance activity. These enzymes were apparently not adsorbed by sintered microporous inorganic spheroids specially fabricated for protein adsorption. The adsorbents removed other proteins, including material shown to be of low molecular weight and assumed to be an inhibitor, permitting considerably enhanced activity.