Expression of the Fungal Cytochrome P450nor cDNA in Escherichia coli
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS(1993)
Abstract
We succeeded in expressing the unique cytochrome P-450 (P-450), nitric oxide reductase (P-450nor), in Escherichia coli by utilizing P-450nor cDNA and the expression vector pKK233-2 or pUC19. The expression was confirmed by Western blot analysis and by detecting the unique nitric oxide reductase activity. The expressed protein was recovered in the soluble fraction of transformed cells, supporting that P-450nor is the first soluble P-450 of eukaryote. The results also provided conclusive evidence that the enzymatic reaction depends solely on P-450nor without support of any other electron transferring components.
MoreTranslated text
Key words
escherichia coli
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined