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27: Progress in Regenerative Medicine Using Cord Blood: Mesenchymal Stem Cell Isolation and Induction to Bone and Cartilage

Biology of Blood and Marrow Transplantation(2007)

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Abstract
Although experimental and clinical trials using bone marrow derived mesenchymal stem cells demonstrated promising results, cells from other sources have also been explored. We report the isolation, expansion and differentiation of mesenchymal stem cells (MSCs) derived from human umbilical cord blood and compare the results to those of MSCs derived from human bone marrow, adipose tissue and chorionic villi of placenta. Though surface antigens of these various MSCs did not show significant differences, differentiation abilities were significantly different among these MSCs. Cord blood-derived MSCs (CB-MSCs) were isolated from 70% of cord blood units processed within 5 hours and at least 60 ml in volume. In each case these MSCs differentiated with high efficiency to chondrocyte lineages as well, or better, than those MSCs derived from bone marrow (BM-MSCs), but adipose tissue-derived MSCs (AD- MSCs) did not. In vivo transplantation of CB-MSCs with scaffolds, atero-collagen for chondrocyte or β-TCP for osteocytes into nude mice resulted in generation of chondrocyte-like tissue as well as bone. Co-culture of MSCs with lymphocytes stimulated with phytohemagglutinin and mixed lymphocyte culture suppressed proliferation of stimulated lymphocytes as well as BM-MSCs and AD-MSCs, indicating that CB-MSCs had immune-suppressive effect. Thus, the CB-MSCs can be a possible allogeneic cell source for regenerative medicine.
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Key words
regenerative medicine,mesenchymal stem cell
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