Molecular Marker Assisted Selecting the Backcross Parents by SSRs for Breeding Cotton Cytoplasmic Male Sterility Lines

By combining traditional breeding selection procedures and molecular marker-assisted methods with SSRs,33 early maturity upland cotton strains for breeding cotton cytoplasmic male sterility lines had been selected.All the 33 strains were clustered into 2 major groups or 4 small categories according to 9 main characters and the amplify results of 260 pairs of SSR molecular markers.The phenotypic characteristics of those subtypes were obviously diffenent.The elite strains with better overall performance of the key characters had been selected from sub-class A1,A2,B1 and B2 which divided by the result of molecular marker.Reffering to the clustering result based on main characters,some of strains which with similar phenotype had been removed.8 typical representative fine strains for backcross breeding cotton CMS lines further have been selected.