Dye sorption as an indicator of erythrocyte membrane damage and prehemolytic state of erythrocytes

High erythrocyte membrane permeability caused by detergents was associated with increased methylene blue sorption, which correlated with erythrocyte damage and preceded hemolysis. The content of sorbed dye in erythrocytes preincubated with 200 μM sodium dodecyl sulfate or cetyltrimethylammonium bromide increased by 58–74% and 2.4–3.8-fold surpassed that in the medium. New precise and convenient method for estimating erythrocyte binding capacity is proposed.