Increased activity of a hybrid Bt toxin against Spodoptera frugiperda larvae from a maize field in Cuba

Biotecnología Aplicada(2006)

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Abstract
Spodoptera frugiperda is the most important maize pest in Cuba. The activity of seven Bacillus thuringiensis Cry1 toxins (five wild-type and two domain-swapped) was investigated in bioassays against newly hatched larvae from adults collected in a Cuban maize field. The CryAAC (1Ac/1Ac/1Ca) hybrid showed the lowest effective dose (50% lethal dose, LD50) of 288 ng/cm 2 contrasting with parental Cry1Ca and Cry1Ac, which were not lethal (LD50 >
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Key words
cryaac,cry δ-endotoxins,bacillus thuringiensis,domain-swapped hybrid toxins,bioassays,spodoptera frugiperda
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